Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones.
نویسندگان
چکیده
A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.
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عنوان ژورنال:
- Journal of lipid research
دوره 7 1 شماره
صفحات -
تاریخ انتشار 1966